{"product_id":"bsa-3-blocking-buffer-1x-in-tbs","title":"BSA (3%) Blocking Buffer 1X in TBS","description":"\u003cp\u003e\u003cstrong\u003eProduct Description\u003cbr\u003e\u003c\/strong\u003eBSA blocking buffer in TBS (1X) is 3% bovine serum albumin (BSA), fraction V, immunology grade, in 1X tris buffered saline (TBS, pH: 7.4) with 0.02% sodium azide. Buffer is used for blocking steps in Western Blot, ELISA, IHC and nucleic acid detection methods. Blocker BSA is usually more effective than nonfat milk blocking buffers for biotin-avidin systems because it contains a single purified protein that is devoid endogenous biotin.\u003cbr\u003e\u003cbr\u003eThe purpose of the blocking step in an assay is to improve assay sensitivity by reducing background interference. Unforeseen cross-reaction of detection reagents with blocking buffers is itself a cause of high background and low signal-to-noise ratios in assay systems. Using inadequate amounts of blocker will result in excessive background. Using excessive blocker concentrations can mask antibody-antigen interactions or inhibit the marker enzyme.\u003c\/p\u003e\r\n\u003cp\u003e\u003c!--[if !supportLineBreakNewLine]--\u003e\u003cbr\u003e \u003c!--[endif]--\u003e\u003c\/p\u003e\r\n\u003cp\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/p\u003e\r\n\u003cul\u003e\r\n\u003cli\u003eAlegria-Schaffer, A., \u003cem\u003eet \u003c\/em\u003e\u003cem\u003eal.\u003c\/em\u003e\u003cspan style=\"font-weight: normal;\"\u003e (2009). Performing and optimizing Western blots with an emphasis on chemiluminescent detection. \u003c\/span\u003e\u003cem\u003eMethods Enzymol.\u003c\/em\u003e\u003cspan style=\"font-weight: normal;\"\u003e 463: 573-99.\u003c\/span\u003e\n\u003c\/li\u003e\r\n\u003c\/ul\u003e\r\n\u003cp\u003e \u003c\/p\u003e","brand":"bioPLUS","offers":[{"title":"100 mL","offer_id":67813178999149,"sku":"fgr1793021540360","price":34.77,"currency_code":"USD","in_stock":true},{"title":"250 mL","offer_id":67813179031917,"sku":"slp1793021540361","price":41.93,"currency_code":"USD","in_stock":true},{"title":"1 L","offer_id":67813179064685,"sku":"ntr1793021540362","price":78.31,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0920\/0526\/9869\/files\/bsa-3-blocking-buffer-1x-in-tbs_1.png?v=1793021544","url":"https:\/\/www.ruralgoods.shop\/products\/bsa-3-blocking-buffer-1x-in-tbs","provider":"My Store","version":"1.0","type":"link"}